Figure 4.

RNR and dNTP production following a DSB is required for efficient HR repair. (A) dTTP levels measured in wild-type, ddb1Δ, and cdt2Δ strains 100 min following 5 μg/mL bleomycin treatment. Means ± standard errors of three experiments are shown. (B) Fivefold serial dilutions of three independently derived cdc22-M45 strains grown on Ye5S. Plates at 33°C and 37°C were incubated for 2 d; the plate at 25°C was incubated for 4 d. (C) Percentage of DSB-induced marker loss in cdc22-M45 grown for 72 h at 33°C. Means ± standard errors of three experiments are shown. The asterisk (*) represents significant difference compared with wild type.