Figure 2.

(A) Scaffold readout of all proteins by spectra of patient 2. MS proteomic data are shown from three samples (1, 2, and 3) prepared from three separate LMDs of Congo red–positive glomeruli. The proteomic data show Igα chain constant region and κ light chains variable and constant region with >95% probability. (B) Representative scaffold readout of proteins of interest for 14 cases of amyloidosis by spectra. The cases are as follows (left to right): four cases of Ig heavy chain amyloid (patients 1 through 4), two cases of fibrogen A-α chain amyloid, two cases of SAA amyloid, four cases of AL amyloid, and two cases of TTR amyloid, respectively. The yellow stars indicate proteins of interest, whereas the red stars indicate protein ambiguity when two proteins share conserved areas.