Table 6.
T cell and Treg cell subsets in 10 of 20 patients with idiopathic MN from RTX administration (baseline) to study end (month 18)
| Parameter | Reference Range | Baseline | Month 1 | Month 6 | Month 12 | Month 18 |
|---|---|---|---|---|---|---|
| CD3+ T cells (total)b | 677 to 2383 cells/μl | 1232 ± 417 | 1466 ± 705a | 1391 ± 603a | 1319 ± 461 | 1306 ± 431 |
| CD3+ CD4+ (helper T cells)b | 424 to 1509 cells/μl | 767 ± 232 | 949 ± 494 | 893 ± 361a | 837 ± 270 | 849 ± 269 |
| CD3+ CD8+ (suppressor T cells)b | 169 to 955 cells/μl | 432 ± 251 | 477 ± 273 | 470 ± 286 | 453 ± 266 | 435 ± 244 |
| CD4+/CD8+ ratiob | ≥1 | 2.33 ± 1.21 | 2.39 ± 1.36 | 2.40 ± 1.36 | 2.49 ± 1.61 | 2.56 ± 1.70 |
| Treg cell subsets | ||||||
| CD4+ CD25+c (activated T cells) | 16 to 323 cells/μl | 228 ± 106 | 259 ± 137 | 253 ± 104 | 177 ± 102 | 226 ± 89 |
| CD4+ CD25+ CD127− CD45 RO+ (natural Tregs)c | 5 to 43 cells/μl | 26.0 ± 13.0 | 24.8 ± 25.1 | 31.7 ± 14.2 | 24.2 ± 11.8 | 28.6 ± 34.9 |
| CD4+ CD25+ CD127− CD45 RA+ (naive Tregs)c | 0.4 to 22 cells/μl | 2.3 ± 3.4 | 2.1 ± 2.1 | 3.7 ± 3.6 | 6.5 ± 5.2 | 10.7 ± 10.0a |
| CD4+ CD25−127+ (Tr1/Th3-induced Tregs)c | 133 to 1304 cells/μl | 533 ± 227 | 508 ± 301 | 617 ± 285 | 509 ± 212 | 548 ± 193 |
Total T cells, CD3+ CD4+ helper cells, CD3+ CD8+ suppressor cells, and CD4+/CD8+ ratio were quantified in whole blood using multicolor flow cytometry in 20 patients with MN. Quantification of T cell subsets became available after the first 10 patients had been recruited. The reference values for CD3, CD4, and CD8 T cells were established from 207 healthy adults (>18 years). The reference values for Tregs were established from 100 healthy adults (>18 years). Mean ± SD.
a
P ≤ 0.05 versus month 0.
b
Mean ± SD of 20 patients.
c
Mean ± SD of 10 patients.