Table 1.
Quantification of the degree of crosslinking between KS and ACP using the program ImageJ. A) Comparison of crosslinking using the panel of analogs with natural KS-ACP partners. B) Comparison of natural versus unnatural KS-ACP partners using analogs 1 and 3. Pantetheine analog used in each experiment is designated in bold.
| A) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |
| KASI-ACP | 87 | 74 | 4 | 5 | 3 | 0 | 0 | 92 | 19 |
| KASII-ACP | 99 | 92 | 27 | 20 | 17 | 4 | 2 | 100 | 100 |
| EncAB-EncC | 42 | 43 | 56 | 23 | 35 | 0 | 0 | 24 | 59 |
| B) | ||
| 1 | 3 | |
| KASI-ACP | 95 | 7 |
| KASI-EncC | 89 | 1 |
| KASII-ACP | 99 | 26 |
| KASII-EncC | 1 | 1 |
| EncAB-ACP | 34 | 28 |
| EncAB-EncC | 32 | 49 |