Figure 2. The TAT peptide penetrates the intact HEK-293 and pulmonary artery smooth muscle cells.
Flow cytometry was conducted on HEK-293 (A) or human pulmonary artery smooth muscle (B) cells that were incubated for 30 minutes with 10 μM 5-FAM labeled TAT peptide (no filling under the curve) or peptide free medium (grey filling under the curve). Cells were then trypsinized and resuspended in PBS as a single cell suspension before flow cytometry.