Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: J Mol Endocrinol. 2009 Jul 20;43(6):251–261. doi: 10.1677/JME-09-0053

Figure 3.

Figure 3

E2 increases association of TrxR with endogenous, estrogen-responsive genes. MCF-7 cells were treated with ethanol (light gray bars) or 10 nM E2 for 0.75 (dark gray bars) or 24 h (black bars) and subjected to ChIP analysis with an (A) ERα-or (B) TrxR-specific antibody. Quantitative real-time PCR was used to examine the association of ERα and TrxR with the ERE-containing regions of the pS2 and PR (PR205 and PR221) genes. Data are presented as the number of copies of each estrogen-responsive gene region pulled down relative to the number of copies of 36B4 gene region pulled down (occupancy). A significant increase in the association of ERα and TrxR with these gene regions in the presence of E2 is indicated by an. asterisk (*p≤0.05).

HHS Vulnerability Disclosure