Table 1. Sequences of primers and probes used for RT-qPCR assay.
| Gene symbol (accession number) | Primer sequence (5′-3′) | Amplicon (bp) |
|---|---|---|
|
S100A4 (NM_011311) |
F- GGACAGCAACAGGGACAATGA |
101 bp |
| |
R- TATCTGGGCAGCCCTCAAAG |
|
| |
P- AGTACTGTGTCTTCCTGTCCTGCATTGCCA |
|
|
S100A6 (NM_011313) |
F- GTACTCTGGCAAGGAAGGTGACA |
101 bp |
| |
R- CAGCATCCTGCAGCTTGGA |
|
| |
P- AAGGAGCTGAAGGAGTTGATCCAGAAG |
|
|
S100A8 (NM_013650) |
F- CGAAAACTTGTTCAGAGAATTGGA |
81 bp |
| |
R- ACTTTTATCACCATCGCAAGGAA |
|
| |
P- ATCAATAGTGACAATGCAATTAACTTCGA |
|
|
S100A9 (NM_009114) |
F- GAGCGCAGCATAACCACCAT |
101 bp |
| |
R- TCCACCATTTGTCTGAATTCCTT |
|
| |
P- ATCGACACCTTCCATCAATACTCTAGGA |
|
|
S100A13 (NM_009113) |
F- CTCAAGGACGTGGGCTCTCT |
101 bp |
| |
R- AGCTCTCCAATCAGTCTCCAGTATTC |
|
| |
P- ATGAAAAGATGAAGACCTTGGATGTGA |
|
|
IL-1β (NM_008361) |
F- AGATGAAGGGCTGCTTCCAA |
81 bp |
| |
R- TGATGTGCTGCTGCGAGATT |
|
| |
P- TGACCTGGGCTGTCCTGATGA |
|
|
IL-6 (NM_031168) |
F- GTTGCCTTCTTGGGACTGATG |
91 bp |
| |
R- TGGGAGTGGTATCCTCTGTGAA |
|
| |
P- TGACAACCACGGCCTTCCCTACTTCA |
|
|
MIP-2 (NM_009140) |
F- GAACATCCAGAGCTTGAGTGTGA |
86 bp |
| |
R- CTTTTTGACCGCCCTTGAGA |
|
| |
P- CCCCAGGACCCCACTGCGC |
|
|
VEGFA (NM_009505) |
F- GCTACTGCCGTCCGATTGAG |
86 bp |
| |
R- CACACAGGACGGCTTGAAGA |
|
| |
P- CCTGGTGGACATCTTCCAGGAGTACCC |
|
|
FGF2 (NM_008006) |
F- AGAGCGACCCACACGTCAA |
86 bp |
| |
R- AAGGTACCGGTTGGCACACA |
|
| |
P- TCCAAGCAGAAGAGAGAGGAGTTGTGT |
|
|
TGFβ1 (NM_011577) |
F- ACGGAATACAGGGCTTTCGA |
86 bp |
| |
R- GCTGATCCCGTTGATTTCCA |
|
| |
P- TCAGCGCTCACTGCTCTTGTG |
|
|
RPL5 (NM_016980) |
F- GGAAGCACATCATGGGTCAGA |
70 bp |
| |
R- TACGCATCTTCATCTTCCTCCATT |
|
| P- TGTGGCAGACTACATGCGCTACC |
In the “Primer” column, “F” indicates forward primer, “R” indicates reverse primer, and “P” indicates probe sequence.