Table 1.
Condition | Intracellular amount of [3H]taurine remained (% of time 0) | |
---|---|---|
TR-TBT 18d-1 | TR-TBT 18d-2 | |
Control | 100 ± 2 | 100 ± 3 |
Ca2+ free | 72.1 ± 3.3 ** | 75.7 ± 4.5 ** |
Cells were incubated for 60 min at 37°C with ECF buffer containing [3H]taurine (28 nM). The cells were washed and then incubated with ECF buffer or Ca2+ free buffer for 30 min Under Ca2+ free condition, Ca2+ was replaced with Mg2+. Remaining of [3H]taurine in the cells was measured. Each value represents the mean ± SEM (n=3-4). ** p<0.01 significantly different from normal efflux.