Figure 2.
Effect of β-alanine-mediated taurine depletion on wobble uridine modification (A) The primer extension method was used to detect levels of 5-taurinomethyluridine in mitochondrial tRNALeu(UUR) before and following β-alanine-treatment. Shown is a representative gel of reverse transcription from the primer. A defect in uridine modification resulted in the extension of reverse transcription through the unmodified uridine (U*), which terminated at cytosine (C) due to the inclusion of dideoxyguanosine (ddGTP) in the reaction mixture. (B) Exposure of cardiomyocytes to β-alanine at various time intervals did not cause a significant change in either modified (τm5U) or unmodified uridine (U).