Fig. 5.

c-Rel is not required for maintenance of tetramer⁺ cells. (A) Representative plots of splenocytes from WT (top row) and c-Rel^−/− mice (bottom row) immunized 3 months prior with 10⁵ CPS-OVA. (B) Frequency of tetramer⁺ cells in the spleens of WT and c-Rel^−/− mice showing combined results from four separate experiments and time points ranging from 60 to 90 days after immunization. (C) Overlays showing CD8⁺ T cells from the spleen of naive mice (shaded gray histogram) compared with WT (black line, top row) or c-Rel^−/− (black line, bottom row) OVA-tetramer⁺ cells. Representative staining from one mouse per group 75 days following immunization is shown; experiment was repeated at least three times with similar results. (D) CD127 expression on WT and c-Rel^−/− tetramer⁺ cells.