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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Arthritis Rheum. 2010 Jul;62(7):1921–1932. doi: 10.1002/art.27470

Figure 1. Microarray analysis of ankle tissue, SF and PBLs during serum-transferred arthritis.

Figure 1

(A) Kinetics of arthritis in B6 mice used for the microarray analysis, represented as mean ankle thickening (in mm) over time. The time-points at which samples were collected are indicated by dashed lines. n=3–4 samples per time-point per sample type (except n=2 for ankle day 1 and PBL day 18); each sample represents pooled RNA from 2–3 mice. (B) Two-time-point fold-change (TTFC) – in which fold-changes from day 0 for consecutive time-points (e.g. days 1 and 3, days 3 and 7, etc.) were averaged and the greatest such value used as a metric – was calculated for each gene on the Affymetrix microarray. The distribution of TTFCs for each of the three sample types is shown, with the vertical axis showing the number of genes with a TTFC equal to or greater than a given TTFC plotted on the horizontal axis. Comparison of the collected data (“Real Data”) is made to an aggregate of 100 randomly generated datasets (“Random Data”). Dashed lines indicate cut-off TTFC values for an estimated false-discovery rate (FDR) of 0.1 in the ankle and synovial fluid datasets.