Table 3.
Concentration of hepatic and fecal bile acids, sterols and activities of lipogenic and lipolytic enzymes in rats fed with methanolic seed extract (50%) of Celastrus paniculatus
| Parameters | Animal groups | ||
|---|---|---|---|
| Group A (Control) |
Group B Hypercholesterolemic |
Group C Hypercholesterolemic plus 50% extract |
|
| Hepatic bile acids (mg/100 g) | 36 ± 2.3 | 40.6 ± 3.3 | 70.6 ± 1.5# |
| Hepatic neutral sterols (mg/100 g) | 100.3 ± 3.5 | 105.6 ± 4.5 | 135.2 ± 3.2# |
| Fecal bile acids (mg/rat/day) | 35.9 ± 1.3 | 38.3 ± 2.5 | 51.3 ± 2.1# |
| Fecal neutral sterols (mg/rat/day) | 90.5 ± 2.6 | 98.8 ± 3.1 | 123.5 ± 3.2# |
| Plasma LCATa | 28.5 ± 0.6 | 27.9 ± 0.3 | 39.4 ± 0.8# |
| Lipoprotein lipaseb | 40.6 ± 1.2 | 35.3 ± 1.2 | 56.6 ± 1.6# |
| Glucose 6-phosphate dehydrogenasec | 64.2 ± 1.3 | 100.3 ± 3.2 | 62.2 ± 1.2# |
| Malate dehydrogennased | 501.3 ± 8.6 | 990.3 ± 9.6 | 453.2 ± 9.6# |
| HMG CoA reductase (ratio of HMG CoA to mevalonate)e | 4.1 ± 0.2 | 6.8 ± 03 | 3.9 ± 0.3# |
Control group (A) and group B compared with 50% ethanolic extract fed group. # P < 0.05
a% increase in the ratio of ester cholesterol to free cholesterol during incubation
bμ moles of glycerol liberated/h/g protein
cOne unit is defined as that amount of the enzyme that causes an increase of 0.01 in optical density/mg protein
dOne unit is defined as that amount of the enzyme that causes an increase of 1.0 in optical density/mg protein [3]
eDecreased ratio indicates increased activity [20]