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. Author manuscript; available in PMC: 2010 Nov 30.
Published in final edited form as: J Anal Toxicol. 2008 Apr;32(3):201–207. doi: 10.1093/jat/32.3.201

Figure 2.

Figure 2

WCS does not affect HBEC integrity. Viability (A). All cultures (control cultures, cultures 10 min after undiluted WCS exposure and WCS-exposed cultures after addition of 70% ethanol) were stained with 5 μM propidium iodide, which fluoresces upon binding to DNA of dead cells (all images are representative from n = 4 cultures per group). Integrity (B). Calcein-stained live HBECs imaged by confocal microscopy pre- (top) and post- (bottom) 10-min WCS exposure (all images are representative from n = 4 cultures per group). Morphology (C). Control HBECs and HBECs exposed to WCS for 10 min were fixed in 1% osmium tetraoxide suspended in perfluorocarbon and stained with Richardson’s stain before being imaged by light microscopy with 100× lens.