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Concentrations of nicotine and cotinine in ASL (in vitro) after cigarette smoke exposure. All cultures were exposed to WCS under thin-film conditions then ASL obtained by lavage. Nicotine in WCS (336.8 ± 10.4 μM); 1:3 (261.3 ± 1.2 μM); 1:10 (40.9 ± 0.1 μM); 1:30 (37.1 ± 0.2 μM); 1:100 (11.8 ± 0.5 μM) (A). Cotinine in WCS (3.2 ± 0.006 μM); 1:3 (2.6 ± 0.02 μM); 1:10 (0.6 ± 0.008 μM); 1:30 (0.5 ± 0.01μM); 1:100 (no peak) (B). n = > 4 for all conditions.
