Figure 2.

Comparable transfection efficacy among cells of HeLa, CaSki and C33A. A total of 1 × 10⁶ cells (Caski or HeLa or C33A) were seeded into a 100-mm² dish and incubate for 16 h. The cells were then transfected with 6 μg of pGFP plasmid for 12 h. The medium was then replaced with fresh medium and normal cell culture was resumed. After 24 h, the cells were examined by a fluorescent microscopy (Nikon, Eclipse, TE300, Nikon Precision Taiwan, Taipei, Taiwan) with visible light (upper panel) or fluorescent light (lower panel).