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. 2010 Oct 1;17(12):827–836. doi: 10.1038/cgt.2010.38

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Copyright © 2010 Nature America, Inc.

This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Colony formation was inhibited in HPV-positive cervical cancer cells by HPV-siRNAs. A total of 1000 cells (Caski or HeLa or C33A) transfected with a specific HPV-siRNA plasmid were seeded in six-well plates and allowed to grow for 10 days. The identity of each HPV-siRNA is indicated at the top of each plate. Relative levels of colony formation were quantified and are shown below each plate. (a) Suppression of colony formation by HPV-16-siRNAs in Caski cells. (b) Suppression of colony formation by HPV-18-siRNAs in Caski cells. (c) Minimal effect of colony formation by HPV-siRNAs in non-HPV infected C33A cells.