Figure 6.
Suppression of tumor growth in xenografts of HPV-positive cervical cancer cells by HPV-siRNAs. BALB/c null mice (seven in each group) were injected with 5 × 106 HeLa cells subcutaneously to generate solid tumors at two sites. After 3 days, 30 μg of either an HPV-18-siRNA or control vector plasmid in 30 μl of normal saline solution was intra-tumorally injected, followed by booster injections of 15 μg of plasmid twice a week for 2 weeks. The tumors were monitored for a total of 30 days. The tumor volume was calculated as the length x width x height. Red lines: tumor growth status in the mice injected with vector plasmid. Blue lines: tumor growth status in the mice injected with HPV18-siRNA. (a) Effect of 18E6-119 siRNA on tumor growth. (b) Effect of 18E6-674 siRNA on tumor growth.