Table 2. List of primer sequences used for the analysis of HPV E6 or E7 gene expressions by RT-PCR.
HPV gene | Primer sequences |
---|---|
HPV16E6 | F: 5′-AATGTTTCAGGACCCTACGG-3′ R: 5′-TCAGGACACAGTGGCTTTTG-3′ |
HPV16E7 | F: 5′-TTTGCAACCAGAGACAACTGA-3′ R: 5′-GCCCATTAACAGGTCTTCCA-3′ |
HPV18E6 | F: 5′-GCGACCCTACAAGCTACCTG-3′ R: 5′-GTTGGAGTCGTTCCTGTCGT-3′ |
HPV18E7 | F: 5′-GCATGGACCTAAGGCAACAT-3′ R: 5′-TGTTGCTTACTGCTGGGATG-3′ |
Actin | F: 5′-GTGGGGCGCCCCAGGCACCA-3′ R: 5′-CTCCTTAATGTCACGCACGATTTC-3′ |
Abbreviations: HPV, human papillomaviruse; RT-PCR, reverse transcription PCR.