(A-C) Aortic expression of ALK2, ALK1 and VEGF in Apoe-/-and MGPtg/wt;Apoe-/-mice fed chow (CH) or Western (HF) diet, as determined by real-time PCR (A), immunoblotting with densitometry (B), and immunohistochemistry (C).
(D) Expression of ALK1 and VEGF colocalize with expression of vWF in atherosclerotic lesions from Apoe-/-and MGPtg/wt;Apoe-/-mice fed a Western diet.
Asterisks indicate statistically significant differences compared to control (Apoe-/- mice on chow). **<0.01, ***<0.001, Tukey's test.