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. 2010 Nov 30;8(11):e1000547. doi: 10.1371/journal.pbio.1000547

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Binding experiments were performed on a MicroCal VP-ITC instrument. For these experiments, the ligand solution used to fill the syringe was 80 µM and AnSt-D7L1 concentration in the calorimeter cell was 8 µM. Assays were performed at 35°C with successive 10 µL injections. Upper curve in each panel shows the heat for each injection; lower part shows the enthalpies for each injection and data were fit to a single binding site model used to calculate the binding stoichiometry (N), dissociation constant (Kd), enthalpy of binding (ΔH), and entropy of binding (TΔS). Panels: (A) PGD₂, (B) PGE₂, (C) PGF_2α, (D) U51605.