Table 1. Comparisons with experimental parameters.
Parameter | Simulation | Experiments | 7 Cell type | Ref. |
1Glucose uptake (kg s m) | Rat-T1, MR1 | [37] | ||
1Lactate release (kg s m) | Rat-T1, MR1 | [37] | ||
2pO (mmHg) | 7 | 0–20 | Rat-T1 | [37] |
0–40 | MR1 | [37] | ||
20–60 | EMT6/Ro | [38] | ||
3pH | 6.7 | 6.6 | C6, H35 | [39] |
6.96–6.99 | U118-MG, HTh7 | [40] | ||
4 pH | 0.77 | 0.41 | U118-MG | [40] |
0.490.08 | HTh7 | [40] | ||
5Viable cell rim thickness (m) | 155 | 200 | EMT6/Ro | [38] |
14216 | HTh7 | [40] | ||
31028 | U118-MG | [40] | ||
19827 | Col12 | [41] | ||
22526 | HT29 | [41] | ||
6Hypoxic rim thickness (m) | 98 | 4452 | Col12 | [41] |
4452 | HT29 | [41] | ||
Cell cycle distribution (%) | G0/G1 = 57.3 | G0/G1 = 584 | BMG-1 | [42] |
S = 21.6 | S = 191 | |||
G2/M = 21.1 | G2/M = 231 |
Metabolic and histologic parameters in spheroids of approximately 500 m diameter: comparison between a single, large simulation, carried out with the parameters listed in Text S1, and experimental data.
Notes:
Rate of glucose uptake or lactate release per viable spheroid volume (see [37]).
Central pO tension (experiments) or estimated in the centroid (simulations).
pH has been determined in the central region of the spheroids. This corresponds to a sphere of radius m about the centroid of the spheroid.
Difference between environmental pH and pH 200 m below the spheroid surface.
In our simulations the viable cell rim thickness corresponds to the distance between the spheroid surface and the inner shell where only 5% of the cells are still alive. Experimental values have been determined by histology.
These values corresponds to the radius of the necrotic core.
Cell types are as follows: Rat-T1 = T24Ha-ras-transfected Rat1 cells (Rat1 = spontaneously immortalized rat embryo fibroblasts); MR1 = myc/ T24Ha-ras-cotransfected rat embryo fibroblasts; EMT6/Ro = mouse mammary tumor cells; C6 = rat glioma cells; H35 = rat hepatoma cells; U118-MG = human glioblastoma cells; HTh7 = human tyroid carcinoma cells; Col12 = moderately differentiated human colon adenocarcinoma ; HT29 = poorly differentiated human colon adenocarcinoma; BMG-1 = human glioma cells.