Table 1. Comparisons with experimental parameters.

Parameter	Simulation	Experiments	7 Cell type	Ref.
1Glucose uptake (kg s m)			Rat-T1, MR1	[37]
1Lactate release (kg s m)			Rat-T1, MR1	[37]
2pO (mmHg)	7	0–20	Rat-T1	[37]
		0–40	MR1	[37]
		20–60	EMT6/Ro	[38]
3pH	6.7	6.6	C6, H35	[39]
		6.96–6.99	U118-MG, HTh7	[40]
4 pH	0.77	0.41	U118-MG	[40]
		0.490.08	HTh7	[40]
5Viable cell rim thickness (m)	155	200	EMT6/Ro	[38]
		14216	HTh7	[40]
		31028	U118-MG	[40]
		19827	Col12	[41]
		22526	HT29	[41]
6Hypoxic rim thickness (m)	98	4452	Col12	[41]
		4452	HT29	[41]
Cell cycle distribution (%)	G0/G1 = 57.3	G0/G1 = 584	BMG-1	[42]
	S = 21.6	S = 191
	G2/M = 21.1	G2/M = 231

Metabolic and histologic parameters in spheroids of approximately 500 m diameter: comparison between a single, large simulation, carried out with the parameters listed in Text S1, and experimental data.

Notes:

¹Rate of glucose uptake or lactate release per viable spheroid volume (see [37]).

²Central pO tension (experiments) or estimated in the centroid (simulations).

³pH has been determined in the central region of the spheroids. This corresponds to a sphere of radius m about the centroid of the spheroid.

⁴Difference between environmental pH and pH 200 m below the spheroid surface.

⁵In our simulations the viable cell rim thickness corresponds to the distance between the spheroid surface and the inner shell where only 5% of the cells are still alive. Experimental values have been determined by histology.

⁶These values corresponds to the radius of the necrotic core.

⁷Cell types are as follows: Rat-T1 = T24Ha-ras-transfected Rat1 cells (Rat1 = spontaneously immortalized rat embryo fibroblasts); MR1 = myc/ T24Ha-ras-cotransfected rat embryo fibroblasts; EMT6/Ro = mouse mammary tumor cells; C6 = rat glioma cells; H35 = rat hepatoma cells; U118-MG = human glioblastoma cells; HTh7 = human tyroid carcinoma cells; Col12 = moderately differentiated human colon adenocarcinoma ; HT29 = poorly differentiated human colon adenocarcinoma; BMG-1 = human glioma cells.