Table 2. Dye dilution proliferation assay.
Numbers represent the percentage of CD4 and CD8 T-cells that proliferated in response to the antigenic stimulus (DCs pulsed with autologous tumor lysate or DCs pulsed with KLH), using the dye dilution proliferation assay. A“-” indicates that there was no proliferation significantly higher than that observed when unpulsed DCs were used as the stimulus. Values in bold are significantly higher than pre-vaccine values, indicating a response induced by the vaccine. Nd indicates the assay was not done, due to lack of cells.
| Percentage of T-cells proliferating in response to antigenic stimulus (CD4/CD8) | ||||||
|---|---|---|---|---|---|---|
| Tumor lysate |
KLH |
|||||
| Pt. | pre-vax | 1 week post-vax | 3 mos post-vax | pre-vax | 1 week post-vax | 3 mos post-vax |
| 1 | - | 2.0/2.0 | - | - | - | - |
| 2 | - | - | - | - | - | - |
| 3 | 2.8/4.0 | 4.0/1.0 | 3.5/0.3 | - | 1.3/- | 1.3/- |
| 4 | - | - | - | - | -/1.8 | - |
| 5 | nd | nd | Nd | Nd | nd | Nd |
| 6 | - | - | - | - | - | - |
| 7 | -/6.0 | -/7.0 | -/4.0 | - | - | - |
| 8 | -/6.0 | -/4.0 | -/0.6 | -/2.0 | - | - |
| 9 | - | -/2.1 | - | - | 4.0/6.2 | 3.9/1.1 |
| 10 | - | - | - | - | - | - |
| 11 | - | - | - | - | - | - |
| 12 | - | - | 3.4/1.7 | - | 1.1/- | 3.4/- |
| 13 | - | - | - | - | - | - |
| 14 | - | - | - | - | - | - |
| 15 | - | 0.7/0.9 | 0.7/- | - | 2.1/6.1 | 2.8/3.3 |
| 16 | - | - | - | 0.9/1.4 | - | 2.1/0.9 |
| 17 | - | - | - | - | 2.3/- | 3.5/1.2 |
| 18 | nd | nd | Nd | Nd | nd | Nd |
| 19 | - | 2.3/2.7 | -/0.9 | 1.4/- | 7.0/3.9 | 9.0/10.0 |
| 20 | - | - | - | - | 3.3/- | 7.8/0.9 |
| 21 | -/0.3 | - | - | - | 0.7/- | 1.4/- |
| 22 | - | - | - | - | 1.0/0.7 | 0.8/- |
| 23 | 1.2/- | 1.7/- | 1.7/- | - | 4.3/1.0 | 2.5/1.7 |
| 24 | - | - | -/0.3 | - | - | - |
| 25 | - | 1.3/- | - | 1.0/- | 8.0/2.6 | 5.0/1.4 |
| 26 | - | 0.4/0.4 | 0.6/- | - | 0.9/0.3 | 0.7/- |