Skip to main content
. 2010 Nov 30;5(11):e14171. doi: 10.1371/journal.pone.0014171

Figure 4. Effect of GABA on α-SMA in fetal lungs: Rat fetuses were injected with GABA receptor modulators.

Figure 4

Immunohistochemistry was done for studying the localization of α-SMA, a marker of myofibroblasts. (A) Shown are the representative images. Higher magnification images indicate specific staining of myofibroblasts in airways (arrow) and vasculature (arrowhead), but not in saccular epithelium (asterisk). Negative controls (non-specific mouse IgG) were devoid of any staining. Scale bar: 40 µm. (B) Five random fields per animal were chosen and quantified for α-SMA localization. Shown are means±SEM of the number of α-SMA positive cells per field. A total of 5 animals were used for the α-SMA staining. *p<0.05 v.s. PBS; #p<0.05 v.s. GABA.