Table 1. Top phosphopeptides identified by peak intensities.
Gene | Peptide | T-test: 2 cGy:C | T-test: 50 cGy:C | 2 cGy/C | 50 cGy/C | Site | PotentialKinase |
SASH1 | R.TCS*FGGFDLTNR.S | 0.0034 | ND | 24.7 | ND | S407 | AKT |
NEXN | K.AEIKEMLAS*DDEEDVSSKVEK.A | 0.0007 | 0.0024 | 13.7 | 21.5 | S80 | CK II |
C11orf58 | R.S*ASPDDDLGSSNWEAADLGNEER.K | 0.0012 | 0.0036 | 21.4 | 14.6 | S15 | None |
BYSL | R.MPQDGS*DDEDEEWPTLEK.A | 0.0108 | 0.2031 | 21.1 | 22.6 | S98 | CK II |
MAP1B | R.DVMS*DETNNEETESPSQEFVNITK.Y | 0.0450 | 0.0140 | 6.8 | 20.2 | S1144 | CK II |
PXN | K.TGSSS*PPGGPPKPGSQLDSMLGSLQSDLNK.L | 0.0016 | 0.0144 | 14.4 | 14.8 | S288 | Erk1 |
GFPT1 | R.VDS*TTCLFPVEEK.A | 0.0239 | 0.0064 | 14.2 | 3.5 | S243 | AMPK |
AHSG | K.CDSSPDS*AEDVRK.V | 0.0428 | 0.0115 | 12.0 | 4.1 | S138 | DNA PK |
PLEC1 | R.TS*SEDNLYLAVLR.A | 0.0092 | 0.0001 | 12.0 | 10.4 | S20 | PKA |
CDC42EP1 | K.NAIS*LPQLNQAAYDSLVVGK.L | 0.0006 | 0.0019 | 8.4 | 11.3 | S121 | None |
ATP2B1 | R.IEDS*EPHIPLIDDTDAEDDAPTK.R | <.0000 | <.0000 | 8.6 | 10.8 | S1193 | CK II |
CFL1 | K.LGGS*AVISLEGKPL.- | 0.0006 | 0.0072 | 10.8 | 10.3 | S155 | None |
LEO1 | K.KYVIS*DEEEEDDD.- | 0.0211 | 0.0024 | 6.2 | 9.6 | S658 | CK II |
TBC1D4 | R.SLTSS*LENIFSR.G | 0.0026 | 0.0016 | 9.0 | 7.3 | S570 | PKC |
SR-A1 | R.FDIYDPFHPTDEAYS*PPPAPEQK.Y | 0.0015 | 0.0027 | 6.3 | 8.8 | S239 | Erk1 |
ZC3H13 | R.NTEESS*SPVRK.E | 0.0379 | 0.2075 | 8.8 | 7.2 | S111 | None |
MAP4 | K.TEVALAKDMES*PTKLDVTLAK.D | 0.0004 | 0.0012 | 8.6 | 6.0 | S280 | Cdk5 |
SEPT2 | K.IYHLPDAES*DEDEDFKEQTR.L | 0.0032 | <.0000 | 7.9 | 8.5 | S218 | CK II |
ARHGEF6 | K.S*TAALEEDAQILK.V | 0.0199 | 0.0177 | 6.3 | 8.3 | S649 | PKC |
ATP2B1 | R.IEDS*EPHIPLIDDTDAEDDAPTKR.N | 0.0043 | 0.0151 | 8.2 | 6.6 | S1193 | None |
MATR3 | R.RDS*FDDRGPSLNPVLDYDHGSR.S | 0.0001 | 0.0013 | 6.1 | 8.2 | S188 | CLK2 |
HDAC1 | R.MLPHAPGVQMQAIPEDAIPEES*GDEDEDDPDKR.I | 0.0368 | 0.0177 | 5.4 | 8.2 | S393 | CK II |
PLEC1 | R.T*SSEDNLYLAVLR.A | 0.0021 | <.0000 | 8.1 | 7.0 | T19 | PKA |
ITGA4 | R.RDS*WSYINSK.S | 0.0334 | 0.0068 | 7.8 | 4.4 | S1027 | PKA |
CEP170 | R.QGS*FTIEKPSPNIPIELIPHINK.Q | 0.0105 | 0.0014 | 2.8 | 7.7 | S838 | CAMK2G |
USP8 | R.SYS*SPDITQAIQEEEK.R | 0.1368 | 0.0092 | 7.8 | 7.2 | S718 | AKT |
EIF5B | K.WDGS*EEDEDNSK.K | 0.0245 | 0.0009 | 7.7 | 4.9 | S164 | CK II |
TP53BP1 | K.MVIQGPSS*PQGEAMVTDVLEDQKEGR.S | 0.0176 | 0.0436 | 6.2 | 7.2 | S1114 | Erk1 |
IFI16 | K.VSEEQTQPPS*PAGAGMSTAMGR.S | 0.0179 | 0.0252 | 0.15 | 0.24 | S153 | ERK1 |
WRNIP1 | K.RPAAAAAAGSAS*PR.S | 0.1212 | 0.020 | 0.36 | 0.096 | S151 | None |
The T-test was used to identify significantly affected phosphopeptides based on relative peak intensities. The complete list can be found in supplementary Table 2.