Table 2. Top phosphopeptides identified by spectral counts.
Gene | peptide | Control | 2 cGy | 50 cGy | 2 cGY GTest | 50 cGY GTest | Site | Potential Kinase |
PRKCDBP | APEPLGPADQSELGPEQLEAEVGES*S*DEEPVESR | 0 | 0 | 30 | ND | 37.856 | S165, S166 | CK II |
PGRMC1 | GDQPAASGDS*DDDEPPPLPR | 16 | 0 | 2 | 19.938 | 9.381 | S56 | CK II |
PRKAR2A | VADAKGDS*ES*EEDEDLEVPVPSR | 38 | 11 | 11 | 16.764 | 11.450 | S77, S79 | CK II |
NOP58 | HIKEEPLS*EEEPCTSTAIASPEK | 0 | 13 | 1 | 15.094 | 0.523 | S502 | ATM |
FAM21B | SPMFPALGEASS*DDDLFQSAK | 0 | 2 | 12 | 1.450 | 13.783 | S1092 | CK II |
GPR124 | ALPAAAEDGS*PVFGEGPPSLK | 11 | 0 | 1 | 13.074 | 7.183 | CDK5 | |
OSBP | MLAES*DES*GDEESVSQTDKTELQNTLR | 0 | 7 | 11 | 7.340 | 12.479 | S190, S193 | CK II |
SSRP1 | EGMNPSYDEYADS*DEDQHDAYLER | 2 | 16 | 13 | 11.324 | 8.912 | S444 | CK II |
RALGPS2 | CHS*LGYNFIHK | 0 | 10 | 2 | 11.181 | 1.456 | S343 | PKC |
PLEKHA5 | TNS*MQQLEQWIK | 0 | 10 | 5 | 11.181 | 4.876 | S410 | PKA |
SRRM1 | TRHS*PT*PQQSNR | 11 | 3 | 0 | 4.890 | 10.706 | S414, T416 | CDK5 |
NIPBL | AITSLLGGGS*PK | 0 | 9 | 0 | 9.893 | ND | S2658 | CDK5 |
BAZ1B | LAEDEGDS*EPEAVGQSR | 8 | 0 | 1 | 9.038 | 4.372 | S1468 | CK II |
AHNAK | AS*LGSLEGEAEAEASSPK | 6 | 22 | 19 | 8.948 | 7.904 | S5749 | PKC |
TJAP1 | GS*PEEELPLPAFEK | 0 | 8 | 4 | 8.615 | 3.680 | S300 | PKA |
JUN | LQALKEEPQTVPEMPGET*PPLS*PIDMESQER | 0 | 8 | 1 | 8.615 | 0.523 | T239, S243 | GSK3 |
MAP1A | WLAES*PVGLPPEEEDKLTR | 0 | 8 | 6 | 8.615 | 6.102 | S1776 | ERK1 |
SORBS3 | LCDDGPQLPTS*PR | 1 | 11 | 3 | 8.603 | 0.957 | S530 | CDC2 |
LMNA | ASSHSSQTQGGGS*VTK | 9 | 2 | 4.700 | 8.463 | S414 | PKC | |
LARP1 | ESPRPLQLPGAEGPAIS*DGEEGGGEPGAGGGAAGAAGAGR | 7 | 0 | 5 | 7.716 | 4.876 | S90 | CK II |
IGF2BP2 | ISYIPDEEVSSPS*PPQR | 7 | 0 | 0 | 7.716 | 6.262 | S164 | CDK5 |
SGPP1 | NS*LTGEEGQLAR | 7 | 0 | 0 | 7.716 | 6.262 | S112 | PKA |
IWS1 | AAVLS*DS*EDEEKASAK | 8 | 2 | 0 | 3.750 | 7.356 | S398, S400 | CK II |
MGMT | GAGATSGS*PPAGRN | 8 | 2 | 0 | 3.750 | 7.356 | S201 | CDC2 |
SNW1 | GPPS*PPAPVMHS*PSR | 8 | 2 | 0 | 3.750 | 7.356 | S224, S232 | ERK1, CDC2 |
IRS2 | TYS*LTTPAR | 0 | 5 | 7 | 4.870 | 7.350 | S577 | AKT |
AKAP12 | VLSKPPEGVVSEVEMLSS*QER | 0 | 1 | 7 | 0.520 | 7.350 | S505 | ATM |
IRF2BP1 | AGGAS*PAASSTAQPPTQHR | 7 | 8 | 0 | 0.030 | 6.262 | S453 | none |
PFKP | GRS*FAGNLNTYK | 7 | 6 | 0 | 0.117 | 6.262 | S386 | AKT |
CHD3 | METEADAPS*PAPSLGER | 7 | 1 | 0 | 4.665 | 6.262 | S1660 | GSK3 |
The G-test was used to identify significantly affected phosphopeptides based on the number of observed spectra. The complete list can be found in Supplementary Table 3.