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. 2010 Nov 30;5(11):e15035. doi: 10.1371/journal.pone.0015035

Figure 1. Actin aggregation induced by GdnHCl in low concentrations.

Figure 1

The dependence of ANS fluorescence intensity (A) and of light scattering (B) of the solutions of inactivated actin (red) and initially native actin after 10 min and 24 h of incubation in a solution of denaturants at the appropriate concentration (blue and green) on GdnHCl (closed symbols) and urea (open symbols) concentration. Insert in panel A. Scheme of actin denaturation and aggregation (N, U* and I are native, essential unfolded and inactivated actin [11], Iag is aggregates of inactivated actin, the details are given in the text). Insert in panel B. The dependence of the total macromolecule charge of actin on the pH of a solution calculated on the basis of protein amino acid content [24]. The protein concentration was 0.15 mg/ml, ANS concentration was 5⋅10−5 M.