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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Biochemistry. 2010 Aug 3;49(30):6485–6493. doi: 10.1021/bi100648w

Figure 2.

Figure 2

Concentration dependence of the observed rate constant for the decarboxylation half-reaction with AC5C (circles) and AC6C (squares) in R406M obtained from analysis of multiwavelength data, the lines represent fits to the Michaelis-Menten equation. Experimental conditions: 20 µM enzyme, 100 mM TEA succinate pH 7.8, 100 mM dipotassium succinate, 20 µM PLP, varying concentrations of AC5C or AC6C.