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Comparison of inhibition of WT (closed circles) and W138F/M141R (open circles) by D-glutamate. The line represents a fit to eq. 2. Inhibition studies were performed using the 2° ADH coupled assay with AIB decarboxylation. Pyruvate was used at a saturating concentration and AIB was held at KAIB while the concentration of D-glutamate was varied. Experimental conditions: 100 mM TEA succinate pH 7.8, 100 mM dipotassium succinate, 20 µM PLP, 1 unit/mL 2° ADH, 300 µM NADPH.
