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. Author manuscript; available in PMC: 2011 Aug 1.

Published in final edited form as: Biochemistry. 2010 Aug 3;49(30):6485–6493. doi: 10.1021/bi100648w

Table 1.

Steady-state Kinetic Parameters for R406 Mutants^a

	AIB				AMS
	k_cat (s⁻¹)	K_AIB (mM)	K_pyr (mM)	k_cat/K_AIB (M⁻¹s⁻¹)	k_cat (s⁻¹)	K_ams (mM)	K_pyr (mM)	k_cat/K_ams (M⁻¹s⁻¹)

R406M	0.22 (0.02)	360 (61)	3.5^b (0.5)	0.6 (0.1)	0.074 (0.004)	452 (73)	3.7 (0.6)	0.16 (0.03)
R406K	1.6 (0.1)	33 (6)	8.8 (0.8)	49 (9)	0.76 (0.05)	62 (11)	6.7 (1.1)	12 (2)
WT	17.7 (0.5)^c	2.2 (0.2)	0.098(0.008)	8000 (800)	4.00 (0.09)	4.5 (0.3)	nd	890 (60)

^a

Errors are given in parentheses. nd, not determined.

^b

K_pyr was determined for the slow form of R406M DGD.

^c

WT values for reaction with AIB taken from (16).