Table 2.
AC5C | AC6C | |||
---|---|---|---|---|
kmax (s−1) |
Kapp (mM) |
kmax (s−1) |
Kapp (mM) |
|
R406M | 9.4 (0.9) × 10−4 | 15 (6) | 5 (1) × 10−4 | 140 (50) |
WTb | 0.98 (0.03)c | 4.0 (0.8) | 2.3 (0.2)c | 53 (150) |
Errors are given in parentheses.
WT values taken from (Sun 37, 3865).
The values given for WT are steady-state kcat and Km values; it is assumed that the decarboxylation half-reaction is rate limiting in WT for AC5C and AC6C, so that kcat = kmax.