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. 2010 Nov 30;5(11):e15069. doi: 10.1371/journal.pone.0015069

Figure 2. DNA methylation levels by MeDIP and the Epityper on 5 promising loci for marker development.

Figure 2

Left panels. The positions of these 5 loci (dotted-line boxes) in relation to the associated genes, and their exons (blocks) and introns (lines) are shown. The genes are shown in the direction of mRNA transcription from left to right. The bar graph below each gene shows the difference in the MeDIP-chip probe signals between the placenta and maternal blood cells plotted against chromosomal location. Each vertical bar represents the signal from one probe. The average inter-probe distance is 35 bp. A positive value implies a higher DNA methylation level in the placenta, compared with maternal blood cells. Horizontal lines with arrows at both ends indicate the regions spanned by the Epityper assays (a–f). Right panels. The median DNA methylation indices (MI) of CpG units within the Epityper assays are shown in a grey intensity scale. Asterisks indicate the CpG units with higher DNA methylation in five placentas than five maternal blood cell samples (Mann-Whitney test, P<0.05). The chromosomal locations and MI of each CpG unit are listed in Table S7.