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. 2010 Nov 30;5(11):e15072. doi: 10.1371/journal.pone.0015072

Figure 2. Representatives of C-di-GMP specific domain architectures in Mycobacteirum tuberculosis and their subsequent protein purification.

Figure 2

(A) Domain architecture of the protein with GGDEF and EAL domain in Mycobacterium tuberculosis. The length of each sequence is approximately to scale. (B) Purification of C- terminal His6 tag proteins Lane – 1 Marker, Lane - 2 MtbDGC and Lane – 3 MtbPDE. C-terminal His6 –tagged Rv1354 is 67.6 KDa and Rv1357c is 33.9 KDa. Purity of the preparations was determined by (10%) SDS- PAGE.