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. 2010 Nov 30;5(11):e15072. doi: 10.1371/journal.pone.0015072

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Gupta et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) LC-ESI-MS/MS m/z 3566.5 (doubly charge), containing disulfide bonded Cys²-Cys⁴⁷⁹ peptide from trypsin and chymotrypsin digested MtbDGC. Inset shows the sequence derived from this MS/MS spectrum. (B) LC-ESI-MS/MS of m/z 1053.2, containing disulfide bonded Cys⁹⁴-Cys⁵⁸⁴ peptide from trypsin and chymotrypsin digested intact unreduced MtbDGC. Inset shows the sequence derived from this MS/MS spectrum. (C) LC-ESI-MS/MS of m/z 2799.1, containing disulfide bonded Cys⁴²⁹-Cys⁶¹⁴ peptide from trypsin and chymotrypsin digested MtbDGC. Inset shows the sequence derived from this MS/MS spectrum.