Table 3. Theoretically and experimental mass of peptides generated from intact proteins samples of MtbDGC upon oxidation.
| Position of Cysteine in Protein | Expected Mass(m/z) | Observed Mass(m/z) | ||
| Unlabelled | Labeled | Unlabelled | Labeled | |
| Digested by Trypsin + Chymotrypsin | ||||
| (Cys2 - Cys479) | 7129.0[(3739.2+3391.8) -2 = 7129.0] | 7129.0 | [M+2H]2+: 3566.5 | [M+2H]2+: 3566.5 |
| (Cys94 - Cys584) | 1053.2[(770.9+284.3) -2 = 1053.2] | 1053.2 | [M+H]+: 1053.2 | [M+H]+: 1053.2 |
| Cys406 | 776.9 | 833.9 | [M+H]+776.9 | [M+H]+: 833.7 |
| (Cys429- Cys614) | 2799.1[(582.6+2218.5) -2 = 2799.1] | 2856.1 | [M+H]+: 2799.1 | [M+H]+: 2856.0 |
| Digested by Chymotrypsin | ||||
| (Cys2 - Cys479) | 9525.7[(4185.7+5342) – 2 = 9525.7] | 9525.7 | [M+3H]3+3176.9 | [M+3H]3+3177.9 |
| (Cys94 - Cys584) | 3847.9[(1257.4+2591.9) – 2 = 3847.9] | 3847.9 | [M+H]+: 3846.2 | [M+H]+: 3846.2 |
| Cys406 | 1396.6 | 1453.6 | [M+H]+1396.0 | [M+H]+: 1453.4 |
| (Cys429- Cys614) | 5259.1[(1107.3+4151.8) – 2 = 4434.2] | 5316.1 | [M+H]+2629.5 | [M+H]+: 2629.5 |
| Digested by Trypsin | ||||
| (Cys2 - Cys479) | 9145.0[(3739.2+4997.7) – 2 = 8734.7] | 8791.7 | [M+3H]3+: 2911.5 | [M+3H]3+: 2911.5 |
| (Cys94 - Cys584) | 244384.0[(2202+2184) – 2 = 4384.0] | 4384.0 | [M+2H]2+: 2461.4 | [M+2H]2+2461.4 |
| Cys406 | 2355.7 | 2412.7 | 2353.0 | 2410.0 |
| (Cys429- Cys614) | 4420.9[(2204.4+2218.5) – 2 = 4420.9] | 4477.9 | [M+H]+:4420.9 | [M+H]+4477.9 |