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. 2010 Nov 30;5(11):e15071. doi: 10.1371/journal.pone.0015071

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García-Faroldi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Bone marrow precursor cells were cultured in vitro into BMMCs as described in Experimental Procedures and maintained until day 35. Granules were then isolated and detection of mMCP-6, COX-IV and acetyl-histone H3 was performed in total cell lysates and in the granule fraction (A) by Western blot. Amines were assayed in the granule fraction (B) by HPLC as described in Experimental Procedures. The chromatogram shown is representative of three independent experiments. Hia, histamine; 5-HT, serotonin; 1,8-DO, 1,8-diaminooctane; Spd, spermidine; Spm, spermine.