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Flow cytometry of MC58 wild-type (a), MC58ΔgapA-1 (b) or MC58ΔsiaD (c) for GapA-1 surface localization. Cells were stained with RαGapA-1 (primary alone), anti-rabbit IgG-Alexa Fluor 488 conjugate (secondary alone) or both. Fluorescence was displayed as a histogram. In panel c, the histogram area in M2 represents the population of fluorescently labelled meningococci.
