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PCR amplification of DNA segments constructed by simultaneous oligo phosphorylation/ligation. Shown are size markers (NEB LMW ladder, lane 1), a 92 bp segment constructed by simultaneous phosphorylation/ligation of precursor octamers (lane 2), which was then joined to a second 92 bp segment constructed by the same approach, to yield a 188 bp product (lane 3).
