Table 3.
Restriction Fragment Length Polymorphism Assays
| Sequence variant | Reagent Role | Reagent | Annealing Temp |
|---|---|---|---|
| g.598T > C2 | Primer | CGAACATGCTTGTCATTCAGT | 58 |
| Primer | ATTTATGGCCTGTCTGGAGGA | ||
| Enzyme | Bcl I | ||
| g.17918T > G2 | Primer | GCAAAATCTTGCCTGTTTCC | 58 |
| Primer | GGGGAGGAGAAGAGGATCAC | ||
| Enzyme | Nsi I | ||
| g.18059C > T2 | Primer | GCAAAATCTTGCCTGTTTCC | 58 |
| Primer | GGGGAGGAGAAGAGGATCAC | ||
| Enzyme | Bsa HI | ||
| g.3C > T3 | Primer | CCACAGAGCCACAGAGCTGCC | 63.7 |
| Primer | GGGCTGGGTGAATTGTGTCATCCT | ||
| Enzyme | Hinc II | ||
| g.1477C > T2 | Primer | GGAGCCTCTTGCCCATAACA | 63 |
| Primer | CTACTACCCTGGATTGAGCG | ||
| Enzyme | Rsa I | ||
| g.1591T > C2 | Primer | CAAATTTGGGTAATGATGTA | 56 |
| Primer | TTCTACTACCCTGGATTGAG | ||
| Enzyme | Mae III | ||
| g.918A > G2 | Primer | CCTGTCGGAGTGTAACCTTGGGC | 63 |
| Primer | TGAGGGAGCTGAGAGGCCAGG | ||
| Enzyme | Bcc I |