Figure 6. Suppressing the ΔF508 phenotype in vivo.
HeLa cells transiently expressing CFTR with the indicated mutations were pulse-labeled with 35S-methionine and cysteine for 15 minutes and chased for the indicated times. CFTR molecules were immunoprecipitated from radiolabeled lysate using polyclonal antibody directed against the R-domain (G449). Samples were analyzed using 7.5% SDS-PAGE. The arrowhead indicates the ER oligomannose form (B-band) and the double arrowhead indicates complex glycosylated CFTR (C-band).