Figure 2.
Retention of HB-IGF-1 on cells lacking heparan sulfate. (A) HB-IGF-1 (HB-IGF) and IGF-1 (IGF) were incubated with mutant CHO cells unable to produce heparan sulfate (pgsD-677) and wildtype CHO cells (WT), then washed in PBS. Western analysis of the cell lysates for IGF showed that HB-IGF-1 remained bound to cells with or without the presence of heparan sulfate, whereas IGF-1 binding was not detectable. (B) Densitometry of Western blots from four repeated experiments, each normalized to wild-type HB-IGF-1 binding. mean ± SEM, * vs. WT HB, p<0.005.