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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Arthritis Rheum. 2010 Dec;62(12):3686–3694. doi: 10.1002/art.27709

Figure 5.

Figure 5

Retention of HB-IGF-1 in human cartilage explants. Human cartilage (grade 0 to 2) was incubated with HB-IGF-1 or IGF-1 for two days (Day -2 to 0), washed, and incubated in IGF-free medium for 4 days (Days 0–4). (A) Amount of HB-IGF-1 or IGF-1 remaining in cartilage after 0–4 days. The experiment was performed on cartilage from four donors and a representative Western blot is shown. (B) Analysis of four Western blots by densitometry, normalized to the density of 5 ng of the respective protein standard. mean ± SEM, * vs. IGF-1, p<0.05.