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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Eur J Neurosci. 2010 Oct 19;32(11):1825–1835. doi: 10.1111/j.1460-9568.2010.07463.x

Figure 1. Ryanodine receptors are functionally expressed in some mouse taste cells.

Figure 1

A, Application of 1μM ryanodine (RY, 30s, light gray column) caused an increase in calcium in isolated mouse taste cells (n=18 out of 53 cells tested). This cell was determined to be a taste cell due to its responsiveness to 10mM denatonium (DEN, 30s, black line). B, RT-PCR analysis of mRNA isolated from circumvallate (C) and foliate (F) taste buds using specific primers for RyR isoforms. Brain mRNA (B) was used as a positive control. PCR amplicons of the correct size were amplified from the brain samples for all three RyR isoforms (RyR1-435 bp, RyR2-635bp, RyR3-505bp), but only RyR1 was amplified from the taste samples. PCR product identity was confirmed using sequence analysis. C, Application of anti-RyR1 to mouse taste buds was visualized using a cy5 secondary antibody. Anti-RyR1 labeling in Cv (top panel) and fungiform taste buds (bottom panel) is shown in the far left panels with an overlay on the bright field image in the middle panels. The negative controls consisting of antibody pre-incubated with the blocking peptide and overlaid on the bright field images are shown in the far right panels. Scale bar = 20μm.