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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Eur J Neurosci. 2010 Oct 19;32(11):1825–1835. doi: 10.1111/j.1460-9568.2010.07463.x

Figure 6. Ryanodine receptors are functionally expressed in dual-responsive taste cells.

Figure 6

A, Application of 1μM ryanodine (RY, 30s) in a dual-responsive taste cell caused an increase in intracellular calcium (n=8 out of 23 cells tested). B, Example trace showing 20μM ryanodine inhibiting the calcium signal due to calcium influx through VGCCs but not the bitter-evoked calcium response in a dual-responsive taste cell (n=5 out of 8 cells tested). C, Pooled results showing the effects of 20μM ryanodine on the calcium signal due to VGCCs in dual-responsive cells (n=13, *P=0.02). D, In dual-responsive taste cells that were sensitive to ryanodine, the Hi K-evoked response was reduced an average of 68% by ryanodine (Inhibited). Compared to dual-responsive taste cells that were not sensitive to ryanodine (Not Inhibited), this was a significantly larger inhibition (Student’s t-test, n=32, ***P<0.0001). E, Calcium responses to 50mM KCl (HiK) were blocked with the VGCC blocker, 200μM CdCl2, to ensure that these elevations were due to opening VGCCs. Cadmium blocked the HiK calcium response that was subsequently inhibited by 20μM ryanodine (RY).