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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Appl Toxicol. 2011 Jan;31(1):1–10. doi: 10.1002/jat.1552

Figure 2.

Figure 2

Effects of Mn and/or LPS on MKK-1/2 activity. Shown are quantification and representative western blots of phosphorylated MKK-1/2 (pMKK-1/2; Ser 218/Ser 222) and total MKK-1/2 in N9 microglia following exposure to vehicle, 250 μM Mn, 100 ng/ml LPS, or 250 μM Mn + 100 ng/ml LPS for 1 (A) and 4 (B) h. Densitometric data were normalized as a ratio of phosphorylated to total MKK-1/2 protein. All data points represent means ± SEM from at least 3 independent experiments. Data were analyzed with ANOVA and means were separated using Fisher’s LSD multiple comparison post hoc test. a Letters denote statistically significant difference from control at p ≤ 0.05.