Specificity of Fli-1 microRNA regulatory element for miR-145.
A. Dose-dependent suppression of Luc-Fli-1-519 by miR-145. 293T cells were transfected with various amounts of miR-145 precursor.
B. Effectiveness of miR-145 inhibitor on luciferase activity of Luc-Fli-1-519. As show, miR-145 inhibitor increased the luciferase activity.
C. Schematic diagram of Luc-Fli-1-3′UTR-Mutation. For Luc-Fli-1-3′UTR-M, eight nucleotides (AACUGGAA) were changed with GGTGATCG. W: Wild; M: Mutation.
D. Reporter mutation analysis. Downregulation of reporter gene with full-length 3′UTR from Fli-1 was apparent, whereas no effect on the Luc-Fli-1-3′UTR-Mutation was detected.
All experiments were performed in triplicates and are shown as mean ± SD. *: P<0.05.