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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Am Chem Soc. 2010 Aug 17;132(48):17053–17055. doi: 10.1021/ja104204z

Figure 2.

Figure 2

Transplantation strategy for incorporating oriented gp10 phi29 DNA motor into C20BAS bolalipid vesicles. A: Surface modification of glass with Ni2+:NTA-PEG600; B: Immobilization and orientation of N-his6-gp10 connector protein via Ni2+:NTA-PEG600 chelation; C: Transplantation of oriented N-his6-gp10 connector protein assembly into C20BAS vesicles via surface-mediated reconstitution; D: Release of immobilized C20BAS vesicles via Ni2+ stripping with imidazole. Final assembly of a functional phi29 motor would involve docking pRNA and gp16 ATPase with the vesicles obtained from D.