Skip to main content
. 2010 Jul 25;38(21):7458–7471. doi: 10.1093/nar/gkq609

Figure 2.

Figure 2.

PPARγ directly regulates SIRT1 transcription. (A) Western blot analysis of SIRT1 and PPARγ expression in vector-transfected and PPARγ-transfected or NC-transfected and PPARγsiRNA-transfected HeLa cells with 20 μM troglitazone or DMSO (vehicle) for 48 h. Western blotting was performed using specific antibodies against SIRT1 and PPARγ as indicated. β-Actin or GAPDH was used as a loading control; NC, negative control. (B) Real-time PCR analysis of SIRT1 expression in NC-transfected and PPARγsiRNA-transfected HeLa cells with20 μM troglitazone or DMSO (vehicle) for 48 h. Each experiment was performed at least three times. Each bar depicts data from three independent PCR reactions (mean ± SD). (C) HeLa cells were transfected with expression plasmids pcDNA3.1 (vector) or pcDNA–PPARγ (PPARγ) together with wild-type SIRT1–Luc or mutant SIRT1–Luc. (D) HeLa cells were transfected with NC and PPARγsiRNA together with wild-type SIRT1–Luc. Cells were subsequently treated with 20 μM troglitazone. Luciferase activity was then measured 48 h after treatment. Values are the mean ±.SD of triplicate data points from a representative experiment (n = 3), which was repeated three times with similar results.