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. 2010 Jul 17;38(21):7673–7688. doi: 10.1093/nar/gkq637

Figure 2.

Figure 2.

GAM modulates E2F1 and Ras levels, increases cell apoptosis and reduces cell proliferation. (A) HEK-293 cells were transfected with either pCMV-HA or HA-GAM-2. GAM effects on E2F1 and Ras were analyzed by western blotting. (B) HEK-293 cells were transfected with either pCMV-HA or HA-GAM-4 along with a control RNA or pre-miR-92a-1. GAM effects on Ras were analyzed by western blotting. (C) Ras levels in HEK-293 cells transfected with either a pre-miR-control, pre-miR-92a-1 or a miR-92a antisense inhibitory RNA (92-I) were determined by western blotting. (D) HEK-293 cells transfected as indicated were assayed 24 h later for apoptosis or proliferation. Values represent the mean ± SD (n = 3). # and ##, Significantly different from apoptosis control, #P = 0.063, ##P < 0.005. * and **, Significantly different from proliferation control, *P < 0.02, **P < 0.005.

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