Table 1.

Product distribution observed after 5′-pyrophosphorylation of trimer RNA

Entry	Reagent	Reaction time (h)	HPLC peak area (%)						Yield (%)
			5′-Pyrophosphate (14.3 min)a	5′-Monophosphate (11.7 min)	By-products
					1 (9.1 min)	2 (12.8 min)	3 (19.2 min)	Others
1	3	1	76.0	3.6	1.9	4.0	2.6	11.9	84
2	4	2	65.0	7.5	4.9	7.9	4.7	10.0	72
3	5	1	55.5	26.4	5.0	0.8	2.0	10.3	61
4	6	1	71.3	5.0	7.0	1.2	5.4	10.1	78
5	7	0.25	2.7	6.9	35.1	2.6	26.9	25.8	3

^aThe numbers in parentheses are the HPLC retention times.