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Masses of 5′-end fragments produced by MazF digestion
| RNA molecule | Peak no.a | Pyrophosphate |
Peak no.a | Cap 1 |
Mass difference (b−a) | ||
|---|---|---|---|---|---|---|---|
| Calculated mass | Observed mass (a) | Calculated mass | Observed mass (b) | ||||
| 130-mer | 1 | 3734.6 | 2 | 4107.4 | 372.8 | ||
| 170-mer | 3 | 3734.2 | 3756.7 | 4 | 4107.4 | 4130.2 | 373.5 |
| 127-mer | 5 | 3734.7 | 6 | 4107.7 | 373 | ||
aPeak numbers correspond to the numbered fragments shown in Figure 6.
